RNA

Part:BBa_K2611002

Designed by: Yuanhan Yang   Group: iGEM18_SCU-China   (2018-10-17)


sgRNA (spacer J23101-RFP)

This sgRNA targets part "spacer J23101-RFP"(BBa_K2611003), and can combines with it's spacer sequence. This sgRNA here can combine with dCas9 protein to direct the complex to the upstream sequence of promoter of RFP to prevent transcription initiation. So, we suggest to use both this part and the RFP we have already modified (BBa_K2611003).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None